Enterobacter aerogenes unknown lab report. How to Write a Microbiology Unknown Lab Report 2019-02-10

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Micro Unknown Lab Report

enterobacter aerogenes unknown lab report

The importance of identifying the genus and species of bacteria is demonstrated by the second unknown. Bacteria, Bacterial cell structure, Cell 861 Words 4 Pages Case Study Report for Unknown 49 A new school for girls was opened in a larger village in Northern Pakistan, and the teachers were proud to have convinced many families in the surrounding villages to allow their daughters a basic education. There were no problems encountered with finding this conclusion. Also, two more nutrient agar plates were used to grow the isolated colonies separate from one another for quality control. A casein test, as described above, was then the performed on Unknown B. Understanding specific bacterial characteristics can influence these choices greatly. If there is no color change, meaning the Simmons Citrate slant stayed green after incubation, there a negative result meaning citrate is not utilized.

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Microbiology Unknown Lab Report

enterobacter aerogenes unknown lab report

The organisms showed weak motility, proving that the unknown organism was flagellated and can produce movement. Despite carbapenem therapy, the patient developed multiple abcesses in the abdominal region, eventually leading to a second liver transplant two months later, at which time carbapenem resistant E. This result was confirmed by the Lab instructor. Introduction In a lab today, I am to identify an unknown bacterium that is causing. This particular protein appears to reduce the production of porins on the gram-negative bacteria, leading to decreased sensitivity to β-lactam antibiotics and therefore might play a role in cell invasion of the host 7.

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Gram Negative Unknown Lab Report Enterobacter Aerogenes Free Essays

enterobacter aerogenes unknown lab report

Isolated pure cultures grew and a gram stain was performed. Utilizing a variety of differential testing and staining methods learned in the microbiology course, students were to determine the identity of an assigned unknown organism. Microbiology Laboratory Theory and Application. . At that time, the bacterial growth was noted and its characteristics were recorded. One organism is known to be a gram positive, and the other a gram negative. Also knowledge of different bacteria helped others.

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Unknown Lab Report, Microbiology

enterobacter aerogenes unknown lab report

Nitrate test was performed, test showed to be positive after the reagents were added. The bacteria being utilized in this experimentation will be unknown gram negative bacterium number 22 that had been inoculated in a Tryptic Soy Broth. The test tubes were labeled number 122 and Alt 5. The purpose of this test was to determine if the bacterium could ferment mannitol McDonald et al. This demonstrates the importance of proper technique in performing the selective tests for bacterial identification. A Gram stain was performed on a sample of the colonies that had grown.

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Micro Unknown Lab Report

enterobacter aerogenes unknown lab report

It has been found to live in various wastes, soil, water, dairy products and hygienic chemicals. This Gram stain demonstrated Gram negative rods and Gram positive cocci. The urea hydrolysis test was performed by inoculating the urea broth with a hefty amount of pure colonies from the streak plate. The concepts used to identify the unknowns in this laboratory activity have a very important place in society today. Then the next steps followed were to find out, what kind of gram positive and gram negative are they based on the chart provided by the instructor. Color change from yellow to pink 1. Once the gram staining was complete, and the gram positive and gram negative were identified, testing went quickly.

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How to Write a Microbiology Unknown Lab Report

enterobacter aerogenes unknown lab report

For healthcare providers it is very important to be aware of what organisms are pathogenic and cause a disease and, therefore, to find an appropriate treatment. In order to tell whether or not an organism tests positive for citrate utilization, Bromthymol blue dye is added to the agar. Microbes and People an A-Z of Microorganisms in Our Lives. Practicing these techniques gives students experience with scientific investigation and report. The Simmons citrate had a negative reaction verifying the gram positive bacteria is a cocci, which eliminates Bacillus cereus and Bacillus subtilis.

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How to Identify Enterobacter aerogenes

enterobacter aerogenes unknown lab report

Indication of gram + bacteria. The recognition of these unknown bacterial cultures is achieved by the isolation and differentiation of the unknown culture based on certain biochemical characteristics, and metabolic pathways. The urea hydrolysis test can yield four possible results: rapid urease-strong urease production-positive, slow urease-weak urease production- weak positive, and urease absent-no urease hydrolysis-negative bacteria. Louis Community College at Meramec, 2011. The Voges-Proskauer test to detect organisms that are able to ferment glucose, but convert the products to acetoin and 2,3-butanediol.

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Unknown Lab Report, Microbiology

enterobacter aerogenes unknown lab report

An inoculating loop was used for proper inoculation of the bacteria. This is important in the medical field because identification of unknown bacteria can help treat a patient by knowing the contributing source of a disease. The purpose of this test was to determine whether or not the bacterium could utilize maltose as its sole carbon source McDonald et al. After observation, the H2S test had a negative result, which pointed to Escherichia coli. Proteus is also found in multiple environmental habitats, including long-term care facilities and hospitals. A selection of enteric bacteria like E.

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Micro Lab Report

enterobacter aerogenes unknown lab report

With the knowledge gained from the sessions in the laboratory, we can now integrate what we have learned to the process of finding out the unknowns given. The unknown bacteria is Staphylococcus aureus. A series of biochemical tests were performed based on the results of the Gram stains. They are found on our skin, in our bodies, in the soil, in our food, in the air, and in water. Second, utilizing those techniques allowed for the identification of two unknown bacteria. The results of the Gram stain determined which biochemical test to use in order to identify the microorganisms.

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Unknown Lab Report, Microbiology

enterobacter aerogenes unknown lab report

This was thought to be a positive reading for mannitol fermentation, until the instructor described about its potential inaccuracy due to it being an isolation attempt. They would be in the Results section. After results were seen, a milk agar plate was then obtained and a casein test was performed according to the lab manual. In this experiment I performed many different test dealing with things such as the presence of enzymes, fermentation abilities and different chemical reactions. The newly streaked plate was placed in the incubator. The majority of the E.


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